Coding
Part:BBa_K4821004:Design
Designed by: Madeline Ho Group: iGEM23_Washington (2023-10-12)
RdhANp_M132P_K12
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1628
Illegal BamHI site found at 312
Illegal BamHI site found at 419 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence was codon-optimized for expression in E. coli K-12 and checked for illegal restriction enzyme sites. Protein was docked to PCB 132 using Rosetta and LigandMPNN.
Source
Wild-type protein: https://www.rcsb.org/structure/6ZXX Wild-type DNA sequence: https://parts.igem.org/Part:BBa_K4821002