Coding

Part:BBa_K4821004:Design

Designed by: Madeline Ho   Group: iGEM23_Washington   (2023-10-12)


RdhANp_M132P_K12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1628
    Illegal BamHI site found at 312
    Illegal BamHI site found at 419
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was codon-optimized for expression in E. coli K-12 and checked for illegal restriction enzyme sites. Protein was docked to PCB 132 using Rosetta and LigandMPNN.



Source

Wild-type protein: https://www.rcsb.org/structure/6ZXX Wild-type DNA sequence: https://parts.igem.org/Part:BBa_K4821002

References